LOCUS       sequence-115004-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses FLAG-HA-tagged HuD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-DestFLAGHA_HuD
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             148..174
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    190..214
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg agctcggatc gatccatcga tagtgtggtg gaattctgat cgccgccgcc
      121 atggactaca aggacgacga tgacaagtac ccttatgacg tgcccgatta cgctttgaac
      181 cagatatcaa caagtttgta caaaaaagca ggctccacca tgggaaccaa ttcagtcgac
      241 atggttatga taattagcac catggagcct caggtgtcaa atggtccgac atccaataca
      301 agcaatggac cctccagcaa caacagaaac tgtccttctc ccatgcaaac aggggcaacc
      361 acagatgaca gcaaaaccaa cctcatcgtc aactatttac cccagaatat gacccaagaa
      421 gaattcagga gtctcttcgg gagcattggt gaaatagaat cctgcaaact tgtgagagac
      481 aaaattacag gacagagttt agggtatgga tttgttaact atattgatcc aaaggatgca
      541 gagaaagcca tcaacacttt aaatggactc agactccaga ccaaaaccat aaaggtctca
      601 tatgcccgtc cgagctctgc ctcaatcagg gatgctaacc tctatgttag cggccttccc
      661 aaaaccatga cccagaagga actggagcaa cttttctcgc aatacggccg tatcatcacc
      721 tcacgaatcc tggttgatca agtcacagga gtgtccagag gggtgggatt
//
