LOCUS       sequence-115009-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GFP-tagged HuB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestGFP_HuB
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(160..162,163..165,166..678)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(205..226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(466..485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag
      181 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      241 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      301 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      361 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      421 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      481 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      541 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      601 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      661 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      721 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      781 ctgagcaaag accccaacga gaagcgcgat cacatggtc
//
