LOCUS       sequence-115011-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 7-9 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX3-63
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(398..415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(398..414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(407..429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      478..564
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      656..683
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc tggctggtct
       61 agacgtctcg caggccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga
      121 gcgcttgctt gccgccattg ttgctggcga tagccaccac ttggtccggg gtcaggccat
      181 ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc ttgccgccac
      241 cgttgctggc gatagccacg acctgatcgg gtgtgaggcc atggtcctgg cacagcaccg
      301 gcaacagccg ctgcaccgtt tcgagcgctt gcttgccgcc accgttgctg gcgatagcca
      361 ccacttggtc cggggtcagg ccatggagac gcttaagact ggccgtcgtt ttacaacgtc
      421 gtgactggga aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa
      481 ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      541 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg
      601 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag
      661 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg
      721 tattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattg
//
