LOCUS       sequence-115019-         359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing (N to C terminal) MBP (Maltose Binding 
            Protein), Ntag, and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS011
FEATURES             Location/Qualifiers
     source          1..359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatgatggcc atgttatcct cctcgccctt gctcaccatc ttgtcatcgt catctttgta
       61 gtcgctactg ttttcagtgg tgcgtgctgc cgggatacga tccatgtgtg cgggcgtgcg
      121 gatcggtacg tagccggccg gcggtggtaa aaccttgtaa ccttcaggaa acattgcatc
      181 gagttcctca tctgacagcg gacggttgcg ttcatcaatt tcgcgttccc agcgccatgc
      241 ttgaagctgt tctttataat ccagtttatc gataccacgt ttcaccagac tcaccggcag
      301 cttccaggca ccgcttttat gaaattgaaa atacaggttt tccgaaccgg ctttcttat
//
