LOCUS       sequence-11502-e        4977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Carroll P, Muttucumaru DG, Parish T
  TITLE     Use of a tetracycline-inducible system for conditional expression in
            Mycobacterium tuberculosis and Mycobacterium smegmatis.
  JOURNAL   Appl Environ Microbiol. 2005 Jun . 71(6):3077-84.
  PUBMED    15933004
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Jun . 71(6):3077-84."
COMMENT     Sequence Label: pTACT1
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(192..818)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     723..742
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    840..858
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    926..944
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     terminator      1036..1082
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1228..1247)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1239..2054
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1312..1331)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2386..2974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2875..2894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc gttgtagtgt tgtggtggca
       61 tccgtggcgc ggccgcggta ccagatcttt aaatctagat atccatggat ccagctgcag
      121 gtcgacggta tcgataactc gacatcttgg ttaccgtgaa gttaccatca cggaaaaagg
      181 ttatgctgct tttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
      241 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct
      301 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
      361 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca
      421 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
      481 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
      541 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
      601 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg
      661 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
      721 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg
      781 ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca
      841 ctctatcatt gatagagtta ttttgtcaaa ctagtttttt atttggatcc cctcgagttc
      901 atgaaaaact aaaaaaaata ttgacactct atcattgata gagtataatt aaaataagct
      961 tgatggtacc gagctcgaat tcgaagctta tcgatgtcga cgtagttaac tagcgtacga
     1021 tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1081 atctgttgtt tgtccggcca tcatggccgc ggtgatcagc tagccaacaa agcgacgttg
     1141 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     1201 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     1261 cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1321 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccc
     1381 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1441 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1501 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc
     1561 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1621 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1681 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1741 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataat cttttgccat
     1801 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     1861 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1921 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1981 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2041 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2101 cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     2161 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     2221 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     2281 tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcacta
     2341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2521 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2761 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3121 cgagcgcaac gcgtgcggcc gcacgcgtga gcccaccagc tccgtaagtt cgggcgctgt
     3181 gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg cggcgtgtac
     3241 ggccgccaca gcggctctca gcggcccgga aacgtcctcg aaacgacgca tgtgttcctc
     3301 ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgttccacc accagggctc
     3361 gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat atctgacttg
     3421 gagctcgtgt cggaccatac accggtgatt aatcgtggtc tactaccaag cgtgagccac
     3481 gtcgccgacg aatttgagca gctctggctg ccgtactggc cgctggcaag cgacgatctg
     3541 ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta catcgaggcg
     3601 aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga cgcagcgctc
     3661 cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg caatcgcgcc
     3721 aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac cgaatacgcg
     3781 cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg cgccgtcgat
     3841 ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc ctgggaaacg
     3901 gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct cggcgcgaac
     3961 atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac gccgctaggg
     4021 cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcgtcccgc cctcatgcgg
     4081 atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc cgagtgccac
     4141 gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc ggacagcgag
     4201 gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg catttgggcg
     4261 gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat ctcgcggaag
     4321 ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc cgcgtcagcc
     4381 atgcatggag gcattgctat gagcgacggc tacagcgacg gctacagcga cggctacaac
     4441 cggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg ccgaaggcgc tcgaatcacc
     4501 ggactatccg aacgccacgt cgtccggctc gtggcgcagg aacgcagcga gtggctcgcc
     4561 gagcaggctg cacgccgcga acgcatccgc gcctatcacg acgacgaggg ccactcttgg
     4621 ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg ttaagcgact cggctatcgg
     4681 gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa aggcccacaa cgaagccgac
     4741 aatccaccgc tgttctaacg caattgggga gcgggtgtcg cgggggttcc gtggggggtt
     4801 ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg ctagttttct ggtttgggcc
     4861 atgcctgtct cgttgcgtgt ttcgttgcgc ccgttttgaa taccagccag acgagacggg
     4921 gttctacgaa tcttggtcga taccaagcca tttccgctga atatcgggga gctcacc
//
