LOCUS       sequence-115025-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RFP-tagged HuB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestRFP_HuB
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(400..418)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     668..687
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
       61 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      121 cagcctccgg actctagcgt ttaaacttaa gcttggtacc gagctcggat ccatggtgag
      181 caagggcgag gggtaccatg gtgagcaagg gggtaccatg gtgagcaagg gcgggggtac
      241 caggtgagca agggcgaggg ggtaccatgg tgagcaaggg cgaggaggtc atcaaagagt
      301 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      361 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      421 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      481 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      541 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      601 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
      661 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
//
