LOCUS       sequence-11503-e        5232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5232)
  TITLE     Tetracycline-inducible system for mycobacterium
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTACT13
FEATURES             Location/Qualifiers
     source          1..5232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..95
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    438..456
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             478..1104
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(554..573)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      1210..1256
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1402..1421)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1413..2228
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1486..1505)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2560..3148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3049..3068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagatcttta aatctagata tccatggatc cagctgcaga attcgagctc ggtaccatca
       61 agcttatttt aattatactc tatcaatgat agagtgtcaa tatttttttt agtttttcat
      121 gaactcgagg ggatctggct gagctccacc gcggtggcgg ccgctctaga cgacacatgc
      181 ccagacactg cggaaatgcc accttcaggc cgtcgcgtcg gtcccgaatt ggccgtgaac
      241 gaccgccgga taagggtttc ggcggtgcgc ttgatgcggg tggacgcccg aagttgtggt
      301 tgactacacg agcactgccg ggcccagcgc ctgcagtctg acctaattca ggatgcgccc
      361 aaacatgcat ggatgcgttg agatgaggat gagggaagca agaatggatc caaataaaaa
      421 actagtttga caaaataact ctatcaatga tagagtgtca acaaaaatta ggaattaatg
      481 atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
      541 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      601 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      661 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      721 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      781 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      841 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      901 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      961 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
     1021 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
     1081 cttaaatgtg aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac
     1141 ggtaaccaag atgtcgagtt atcgataccg tcgacgtagt taactagcgt acgatcgact
     1201 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1261 tgtttgtccg gccatcatgg ccgcggtgat cagctagcca acaaagcgac gttgtgtctc
     1321 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1381 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
     1441 gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     1501 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag ccccatgcgc
     1561 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1621 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1681 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
     1741 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1801 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1861 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1921 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taatcttttg ccattctcac
     1981 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2041 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2101 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2161 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2221 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     2281 gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca
     2341 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc
     2401 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc
     2461 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc actagttcca
     2521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2701 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2941 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3301 caacgcgtgc ggccgcacgc gtgagcccac cagctccgta agttcgggcg ctgtgtggct
     3361 cgtacccgcg cattcaggcg gcagggggtc taacgggtct aaggcggcgt gtacggccgc
     3421 cacagcggct ctcagcggcc cggaaacgtc ctcgaaacga cgcatgtgtt cctcctggtt
     3481 ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc caccaccagg gctcgacggg
     3541 agagcggggg agtgtgcagt tgtggggtgg cccctcagcg aaatatctga cttggagctc
     3601 gtgtcggacc atacaccggt gattaatcgt ggtctactac caagcgtgag ccacgtcgcc
     3661 gacgaatttg agcagctctg gctgccgtac tggccgctgg caagcgacga tctgctcgag
     3721 gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca
     3781 acagcgctgg caaacctgct ggtcgtggac gtagaccatc cagacgcagc gctccgagcg
     3841 ctcagcgccc gggggtccca tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc
     3901 cacgcacacg cagtgtgggc actcaacgcc cctgttccac gcaccgaata cgcgcggcgt
     3961 aagccgctcg catacatggc ggcgtgcgcc gaaggccttc ggcgcgccgt cgatggcgac
     4021 cgcagttact caggcctcat gaccaaaaac cccggccaca tcgcctggga aacggaatgg
     4081 ctccactcag atctctacac actcagccac atcgaggccg agctcggcgc gaacatgcca
     4141 ccgccgcgct ggcgtcagca gaccacgtac aaagcggctc cgacgccgct agggcggaat
     4201 tgcgcactgt tcgattccgt caggttgtgg gcctatcgtc ccgccctcat gcggatctac
     4261 ctgccgaccc ggaacgtgga cggactcggc cgcgcgatct atgccgagtg ccacgcgcga
     4321 aacgccgaat ttccgtgcaa cgacgtgtgt cccggaccgc taccggacag cgaggtccgc
     4381 gccatcgcca acagcatttg gcgttggatc acaaccaagt cgcgcatttg ggcggacggg
     4441 atcgtggtct acgaggccac actcagtgcg cgccagtcgg ccatctcgcg gaagggcgca
     4501 gcagcgcgca cggcggcgag cacagttgcg cggcgcgcaa agtccgcgtc agccatgcat
     4561 ggaggcattg ctatgagcga cggctacagc gacggctaca gcgacggcta caaccggcag
     4621 ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta
     4681 tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag
     4741 gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa
     4801 acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg
     4861 aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca
     4921 ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg
     4981 caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct
     5041 gtctcgttgc gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta
     5101 cgaatcttgg tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc
     5161 ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg ttgtggtggc atccgtggcg
     5221 cggccgcggt ac
//
