LOCUS       sequence-115033-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS with R38Q mutation for stable cell 
            line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_R38Q
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             163..186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             187..213
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    229..253
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa ggacgacgat
      181 gacaagtacc cttatgacgt gcccgattac gctttgaacc agatatcaac aagtttgtac
      241 aaaaaagcag gctccaccat gggaaccaat tcagtcgaca tgaacaacgg cggcaaagcc
      301 gagaaggaga acaccccgag cgaggccaac cttcaggagg aggaggtccg gaccctattt
      361 gtcagtggcc ttcctctgga cattaaacct caggagctct atctgctttt cagaccattt
      421 aagggctatg agggttctct tataaagctc acatctaaac agcctgtagg ttttgtcagt
      481 tttgacagtc gctcagaagc agaggctgca aagaatgctt tgaatggcat ccgcttcgat
      541 cctgaaattc cgcaaacact acgactagag tttgctaagg caaacacgaa gatggccaag
      601 aacaaactcg tagggactcc aaaccccagt actcctctgc ccaacactgt acctcagttc
      661 attgccagag agccatatga gctcacagtg cctgcacttt accccagtag ccctgaagtg
      721 tgggccccgt accctctgta cccagcggag ttagcgcctg ctctacctcc tcctgctttc
      781 acctatcccg cttcactgca tgcccagatg cgctggctcc ctccctccga ggctacttct
      841 cagggctgga agtcccgtca gttctgctg
//
