LOCUS       sequence-115034-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing (N to C terminal) Ntag and beta-galactosidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS014
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(10..28,29..34,35..51,52..57,58..82)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    35..53
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             407..430
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             431..604
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(451..468)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(451..467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..482)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agattgacat ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
       61 cacatcagca ggacgcactg acaggtaccg catgtcgatc acgtcgctgt ataagaaagc
      121 cggttcggaa aacctgtatt ttcaatttca taaaagcggt gcctggaagc tgccggtgag
      181 tctggtgaaa cgtggtatcg ataaactgga ttataaagaa cagcttcaag catggcgctg
      241 ggaacgcgaa attgatgaac gcaaccgtcc gctgtcagat gaggaactcg atgcaatgtt
      301 tcctgaaggt tacaaggttt taccaccgcc ggccggctac gtaccgatcc gcacgcccgc
      361 acacatggat cgtatcccgg cagcacgcac cactgaaaac agtagcgact acaaagatga
      421 cgatgacaag atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg
      481 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      541 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      601 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg cc
//
