LOCUS       sequence-115043-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H2B fused to mCherry for chromatin visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     336..353
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        373..391
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     373..390
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(685..704)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     954..972
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      121 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      181 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      241 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      301 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      361 gcgagctcta gcatttaggt gacactatag aatagggccc tctagattac ttgtacagct
      421 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
      481 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
      541 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
      601 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
      661 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
      721 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
      781 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      841 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
      901 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
      961 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     1021 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcg
//
