LOCUS       sequence-115044-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H2B fused to mCherry for chromatin visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..482
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     regulatory      495..504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(646..664)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     914..933
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccgc caccatgcct gaaccctcta
      121 agtctgctcc agcccctaaa aagggttcta agaaggctat cactaaggcg cagaagaagg
      181 atggtaagaa gcgtaagcgc agccgcaagg agagctattc tatctatgtg tacaaggttc
      241 tgaagcaggt ccaccccgac accggcatct catccaaggc catggggatc atgaactcct
      301 tcgtcaacga catcttcgag cgcatcgcgg gcgaggcttc tcgcctggct cactacaata
      361 agcgctcgac catcacctcc agggagattc agacggctgt gcgcctgctg ctgcctgggg
      421 agctggctaa gcatgctgtg tccgagggca ctaaggcagt taccaagtac actagctcta
      481 aggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
      541 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      601 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      661 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      721 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      781 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      841 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
      901 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      961 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     1021 acggcggcca ctacgacgct ga
//
