LOCUS       sequence-115050-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS with K36E/R38E mutation for stable 
            cell line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_K36E_R38E
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    238..262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat ggactacaag
      181 gacgacgatg acaagtaccc ttatgacgtg cccgattacg ctttgaacca gatatcaaca
      241 agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt cagtcgacat gaacaacggc
      301 ggcaaagccg agaaggagaa caccccgagc gaggccaacc ttcaggagga ggaggtccgg
      361 accctatttg tcagtggcct tcctctggac attgaacctg aggagctcta tctgcttttc
      421 agaccattta agggctatga gggttctctt ataaagctca catctaaaca gcctgtaggt
      481 tttgtcagtt ttgacagtcg ctcagaagca gaggctgcaa agaatgcttt gaatggcatc
      541 cgcttcgatc ctgaaattcc gcaaacacta cgactagagt ttgctaaggc aaacacgaag
      601 atggccaaga acaaactcgt agggactcca aaccccagta ctcctctgcc caacactgta
      661 cctcagttca ttgccagaga gccatatgag ctcacagtgc ctgcacttta ccccagtagc
      721 cctgaagtgt gggccccgta ccctctgtac ccagcggagt tagcgcctgc tctacctcct
      781 cctgctttca cctatcccgc ttcactgcat gcccagatgc gctggctccc
//
