LOCUS       sequence-115056-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single vector system expressing NEF allowing myristoylation of 
            recombinant protein produce in Ecoli system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Gluck JM, Hoffmann S, Koenig BW, Willbold D
  TITLE     Single vector system for efficient N-myristoylation of recombinant 
            proteins in E. coli.
  JOURNAL   PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.
  PUBMED    20404920
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-9"; volume: "5(4):e10081. doi"; pages: " 
            10.1371/journal.pone.0010081"
COMMENT     Sequence Label: pETDuet-1∆HisTag_Nef_MCS2
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    99..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(123..142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(124..142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(230..249)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        455..532
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(552..571)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agcattatgc ggccgcaagc ttgtcgacct gcaggcgcgc cgagctcgaa ttcatggtat
       61 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
      121 tcccctatag tgagtcgtat taatttcgcg ggatcgagat cgatctcgat cctctacgcc
      181 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      241 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      301 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      361 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      421 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
      481 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
      541 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      601 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      661 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      721 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      781 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      841 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      901 cattaactat ccgctgg
//
