LOCUS       sequence-115064-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian N. meningitidis Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMcas
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..55)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(68..88)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(92..112)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(116..136)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgtgctggat ctatgcggcc ccattcagat cctcttctga gatgagtttt tgttctgcgg
       61 ccgtatctac ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct
      121 ttctcttctt ttttggatct gcggccgcgc gcacaggagg gcgcttcttc aggcggcagg
      181 ggcggatctc cttgcccagc tcgtcgatct ggtacttctg gaagctcagg gcggtcttca
      241 cgccgatgcc ctccaggatg ccgttcttgc cgatcttgtg gtccaggtcg tggatgcgga
      301 tgttgatgtt gccggtgccg cggtggcagc tggcgaagta gccgaacatg cgggccttct
      361 tggtgatcac ctccaccagg tcgttggggt gcaggctgaa cttgaagttg aagctgtcgt
      421 cgatcagctg ccagtcctcc tcgtccttgc cctgcaccac ggcgcggtcg ggcaggatgc
      481 ccttggccac ctgccagctg tagatgggca ccaggtagta cttgtcgccc ttctcgaaca
      541 cgtccacgcg caccatggtg gcgttgtcgg cgatgccgtt gtggttgcgc acccacacgc
      601 cggtcttctg cacctgctcc acgcgcacgg ccttcacctg ctgggtgcgg ttgccggcct
      661 tgtcgtactt gtagaagggc tcggcgaagg ccttggcggg gtcgtccttg tgggcctcca
//
