LOCUS       sequence-115065-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid mediates polycistronic expression of 4 genes: Ngn3, 
            Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic 
            acinar cells to induced beta-like cells in vivo. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, 
            Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y,
            Zeng S, Gifford D, Meissner A, Weir G, Zhou Q
  TITLE     Long-term persistence and development of induced pancreatic beta 
            cells generated by lineage conversion of acinar cells.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub
            2014 Nov 17.
  PUBMED    25402613
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3082"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1223-30"
COMMENT     Sequence Label: pAd-M3cherry
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..844
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctggctag ttaagctatc aacaagtttg tacaaaaaag caggctggcg ccggaaccaa
      121 ttcagtcgac gccgccacca tggcgcctca tcccttggat gcgctcacca tccaagtgtc
      181 cccagagaca caacaacctt ttcccggagc ctcggaccac gaagtgctca gttccaattc
      241 caccccacct agccccactc tcatacctag ggactgctcc gaagcagaag tgggtgactg
      301 ccgagggacc tcgaggaagc tccgcgcccg acgcggaggg cgcaacaggc ccaagagcga
      361 gttggcactc agcaaacagc gaagaagccg gcgcaagaag gccaatgatc gggagcgcaa
      421 tcgcatgcac aacctcaact cggcgctgga tgcgctgcgc ggtgtcctgc ccaccttccc
      481 ggatgacgcc aaacttacaa agatcgagac cctgcgcttc gcccacaact acatctgggc
      541 actgactcag acgctgcgca tagcggacca cagcttctat ggcccggagc cccctgtgcc
      601 ctgtggagag ctggggagcc ccggaggtgg ctccaacggg gactggggct ctatctactc
      661 cccagtctcc caagcgggta acctgagccc cacggcctca ttggaggaat tccctggcct
      721 gcaggtgccc agctccccat cctatctgct cccgggagca ctggtgttct cagacttctt
      781 gactagtgcc acgaacttct ctctgttaaa gcaagcagga gatgttgaag aaaaccccgg
      841 gcctggatcc atgaacagtg aggagcagta ctacgcggcc acacagctct acaaggaccc
      901 g
//
