LOCUS       sequence-115067-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Clover-mRuby2-FRET-10
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..733
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat ccgctagcca ccatggtgtc taagggcgaa gagctgatca aggaaaatat
       61 gcgtatgaag gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga
      121 aggagaaggc aatccgtaca tgggaactca aaccatgagg atcaaagtca tcgagggagg
      181 acccctgcca tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacttt
      241 tatcaagtac ccgaaaggca ttcctgattt ctttaaacag tcctttcctg agggttttac
      301 ttgggaaaga gttacgagat acgaagatgg tggagtcgtc accgtcatgc aggacaccag
      361 ccttgaggat ggctgtctcg tttaccacgt ccaagtcaga ggggtaaact ttccctccaa
      421 tggtcccgtg atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc
      481 agcagatggt ggtctgaggg gatacactca tatggcactg aaagttgatg gtggtggcca
      541 tctgtcttgc tctttcgtaa caacttacag gtcaaaaaag accgtcggga acatcaagat
      601 gcccggtatc catgccgttg atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat
      661 gttcgtagta caacgcgaac acgcagttgc caagttcgcc gggcttggtg gtgggatgga
      721 cgagctgtac aagtccggac tcagatcccc accggtcgcc accatggtga gcaagggcga
      781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacgg
//
