LOCUS       sequence-115079-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a gRNA that targets Can1.Y in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p426-SNR52p-gRNA.CAN1.Y-SUP4t
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        44..312
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        333..407
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      412..431
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      437..684
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(702..721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(703..721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(731..748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(731..747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(740..762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gcttctttga aaagataatg
       61 tatgattatg ctttcactca tatttataca gaaacttgat gttttctttc gagtatatac
      121 aaggtgatta catgtacgtt tgaagtacaa ctctagattt tgtagtgccc tcttgggcta
      181 gcggtaaagg tgcgcatttt ttcacaccct acaatgttct gttcaaaaga ttttggtcaa
      241 acgctgtaga agtgaaagtt ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa
      301 agataaatga tcgatacgtt ctctatggag gagttttaga gctagaaata gcaagttaaa
      361 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtggtg ctttttttgt
      421 tttttatgtc ttcgagtcat gtaattagtt atgtcacgct tacgttcacg ccctcccccc
      481 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
      541 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
      601 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
      661 ggacgctcga aggctttaat ttgcggccgg tacccaattc gccctatagt gagtcgtatt
      721 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      781 aactta
//
