LOCUS       sequence-115103-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mOrange-H2B-6
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(45..422)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(511..714)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(540..560)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttattctcct cgcccttgct caccatggtg gcgaccggtg gatccttagc gctggtgtac
       61 ttggtgatgg ccttagtacc ctcggacacg gcgtgcttgg ccaactcccc aggcagcagc
      121 aggcgcacgg ccgtctggat ctccctggag gtgatggtcg agcgcttgtt gtaatgcgcc
      181 aggcgggaag cctcacctgc gatgcgctcg aaaatgtcgt tcacaaacga attcatgatg
      241 cccatggcct tggacgaaat gccggtgtca gggtggacct gcttcagaac cttgtacaca
      301 tagatggaat agctctcctt gcggctgcgc ttgcgcttct tgccgccttt cttctgcgcc
      361 ttagtcaccg ccttcttgga gccctttttc ggggcgggag cagacttcgc tggctctggc
      421 atggtggcgg taccgtcgac tgcagaattc gaagcttgag ctcgagatct gagtccggta
      481 gcgctagcgg atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac
      541 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      601 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      661 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      721 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      781 ctgggcataa tgccaggcgg gccatttacc gtcattgac
//
