LOCUS       sequence-115106-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For transfection or retroviral production. Mammalian expression of 
            EGFP driven by the ECMV IRES..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pLPCX-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(314..333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(353..386)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(446..479)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     607..629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      301 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtac tgataacttc
      361 gtatagcata cattatacga agttatatta agggttccgg atcgcggccc tagcttggct
      421 ggacgtaaac tcctcttcag acctaataac ttcgtatagc atacattata cgaagttatc
      481 agtacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
      541 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      601 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
      661 agccagctta cctcccggtg gtgggtcggt ggtccctggg caggggtctc ccgatcccgg
      721 acgagccccc aaatgaaaga cccccgctga cgggtagtca atcactcaga ggagaccctc
      781 ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt ggatacacgg
      841 gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt tgtgggctct
      901 tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac tgattggtta
      961 gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac atctgatggt
     1021 tc
//
