LOCUS       sequence-115116-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GalT reporter only ( no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: flGalT-SBP-mCherry_puromycin
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..227)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatacctgc aggtggttca
      121 cgttgacctt gtgggtgatg ctccagtcgt gcacgaagtt gttcaagttc cccagccagt
      181 ccttcaacaa cgtgtccacc tcgccaacca gtggtcttct cgtccatgga attcaagctc
      241 ggtgtcccga tgtccactgt gatttgggta tacaatgggt atctctgtac atccagcacc
      301 tggtaggtga gtgagttcaa accatcagag agcattgtct cctttgtgtg tgcaattcgg
      361 tcaaacctct gaggattggg ttcatttttc ttgtctcttg agtggcggat catgcgacac
      421 ctcccgacca cagcatttgg gcgagatata gacatgcctc taaaaactaa tctgttaaaa
      481 atgtcatcat cttctcctcc ccagccccaa taattattag gaaatccatt gatggttaga
      541 aactgttgtt tacttagagc agagacacct ccaaaatact gaacataagg taggctgaat
      601 ccaaacttat ccattgcaac ggaaatgtgc cgtggctgtg aaaaacacct gtacgcatta
      661 tggtcattca ttggaatgag gtccacgtca ctaaacacaa agcaggtgta gtcatagtcc
      721 ttcaaggctt cttgaaagcc aacattgagg agcttagcac gattgaatat agtgtctccc
      781 gcct
//
