LOCUS       sequence-115122-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ST reporter only ( no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: ST-SBP-mCherry_puromycin
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             267..380
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(535..553)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatcgatat ctgcggccta gctagcggga
      121 ggcgcgccat gattcacacc aacctgaaga aaaagttcag ctgctgcgtc ctggtctttc
      181 ttctgtttgc agtcatctgt gtgtggaagg aaaagaagaa agggagttac tatgattcct
      241 ttaaattgca aaccaagggg aattccatgg acgagaagac cactggttgg cgaggtggac
      301 acgttgttga aggactggct ggggaacttg aacaacttcg tgcacgactg gagcatcacc
      361 cacaaggtca acgtgaacca cctgcaggta tggtgagcaa gggcgaggag gataacatgg
      421 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      481 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      541 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      601 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      661 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      721 ccgtgaccca ggactcctcc ctacaggacg gcgagttcat ctacaaggtg aagctgcgcg
      781 gcaccaac
//
