LOCUS       sequence-115134-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CREB1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CREB1_2
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     228..249
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(292..345)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(361..408)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
       61 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
      121 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
      181 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      241 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ctgggccagg
      301 attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctccac tgccgaattc
      361 ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt cgcctcccag
      421 ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc
      481 gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt
      541 gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg
      601 ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc
      661 gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta
      721 gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcgggg
//
