LOCUS       sequence-115137-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CREB1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CREB1_2
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
       61 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
      121 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
      181 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      241 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      301 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      361 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      421 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      481 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      541 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      601 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      661 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      721 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      781 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
      841 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
      901 gattgccctg agcctgggcc tgaccccc
//
