LOCUS       sequence-115154-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single vector system expressing hNMT1 (81-496 amino acids) and NEF 
            allowing myristoylation of recombinant protein produce in Ecoli 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Gluck JM, Hoffmann S, Koenig BW, Willbold D
  TITLE     Single vector system for efficient N-myristoylation of recombinant 
            proteins in E. coli.
  JOURNAL   PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.
  PUBMED    20404920
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-9"; volume: "5(4):e10081. doi"; pages: " 
            10.1371/journal.pone.0010081"
COMMENT     Sequence Label: pETDuet-1∆HisTag_hNMT_Nef
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcattatgc ggccgcaagc ttttattgta gcaccagtcc aaccttctct gcccccatgc
       61 tggggcattt ccaattgtaa aggtaatact gcaggttgcc gtcccctatg ccaaacttga
      121 gcttctccag gaaggttttg ttctccatga gatccagtgc attgaacaca tcaaaccctt
      181 tcattttggc gaggacaagg gcgtcgctca tgaggtctag aagaggggtc tgggtgtgaa
      241 cgttgtagaa agaataagca gctttgagac tcttgtgggt tggatggttc atgatggtgg
      301 agggcagcgt ataaaagctc aggaaatctg tcacctctcc gtttgcgttc tccaccacga
      361 aagtgtcgat gatattctcc tgggggtaga accagtgctc cacctcctcc tggctcatga
      421 cgggcgtaag gtgaaattgc ttcaagtacc tggtgaggag ctggtgcact actggaatgt
      481 cctttgtttc cattggtcgc agcccagctg tcttgggagt ctctggcagt cggtagagct
      541 tcatggtgcg ctgcatggtc atatttctgc tcaggtggga gaacttcact tcaatcagct
      601 tccgtgggtt tagggaccga tgccaatacc tgcaggtgcc aacaggcttt ggtagtacca
      661 ccccggcagt gtaaactgct tggaagatgc cctccaggtg aacccgcctg gtgatctctc
      721 ggatcagaac tggagcaacc ctcttgg
//
