LOCUS       sequence-115158-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Streptococcus pyogenes sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SpSrtA
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgtcggt cctgcaagct caaatggccg
       61 ctcaacaact gccggtcatc ggtggtatcg ctatcccgga actgggtatc aacctgccga
      121 tttttaaagg cctgggtaac accgaactga tctatggcgc cggtacgatg aaagaagaac
      181 aggtgatggg cggtgaaaac aattacagtc tggcttccca tcacattttt ggcatcaccg
      241 gtagctctca gatgctgttc agcccgctgg aacgtgcgca aaatggcatg agcatctacc
      301 tgaccgataa agaaaaaatc tacgaataca tcatcaaaga tgtctttacg gtggcgccgg
      361 aacgtgtgga cgttattgat gacaccgccg gcctgaaaga agttacgctg gtcacctgca
      421 cggatatcga agcaaccgaa cgcattatcg ttaaaggtga actgaaaacg gaatacgact
      481 tcgacaaagc accggcggat gttctgaaag catttaatca ttcttacaac caggtctcca
      541 cgggctgcat cacgggagat gcactagttg ccctacccga gggcgagtcg gtacgcatcg
      601 ccgacatcgt gccgggtgcg cggcccaaca gtgacaacgc catcgacctg aaagtccttg
      661 accggcatgg caatcccgtg ctcgccgacc ggctgttcca ctcc
//
