LOCUS       sequence-115159-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KQ
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agaaggagat atacatatgc aagctaaacc tcaaattccg aaagataaat cgaaagtggc
       61 aggctatatt gaaattccag atgctgatat taaagaacca gtatatccag gaccagcaac
      121 acctgaacaa ttaaatagag gtgtaagctt tgcaaaggaa aatcagtcac tagatgatca
      181 aaatatttca attgcaggac acactttcat tgaccgtccg aactatcaat ttacaaatct
      241 taaagcagcc aaaaaaggta gtatggtgta ctttaaagtt ggtaatgaaa cacgtaagta
      301 taaaatgaca agtataagag atgttaagcc tacagatgta gaagttctag atgaacaaaa
      361 aggtaaagat aaacaattaa cattaattac ttgtgatgat tacaatgaaa agacaggcgt
      421 ttgggaaaaa cgtaaaatct ttgtagctac agaagtcaaa ggctgcatca cgggagatgc
      481 actagttgcc ctacccgagg gcgagtcggt acgcatcgcc gacatcgtgc cgggtgcgcg
      541 gcccaacagt gacaacgcca tcgacctgaa agtccttgac cggcatggca atcccgtgct
      601 cgccgaccgg ctgttccact ccggcgagca tccggtgtac acggtgcgta cggtcgaagg
      661 tctgcgtgtg acgggcaccg cgaaccaccc gttgttgtgt ttggtcgacg tcgccggggt
      721 gccgaccctg ctgtggaagc tgatcgacga aatcaagccg ggcgattacg cggtgattca
      781 acgcagcgca ttcagcgtcg actgtgcagg tt
//
