LOCUS       sequence-115160-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KA
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgaaagata aatcgaaagt ggcaggctat attgaaattc cagatgctga tattaaagaa
       61 ccagtatatc caggaccagc aacacctgaa caattaaata gaggtgtaag ctttgcaaag
      121 gaaaatgcct cactagatga tcaaaatatt tcaattgcag gacacacttt cattgaccgt
      181 ccgaactatc aatttacaaa tcttaaagca gccaaaaaag gtagtatggt gtactttaaa
      241 gttggtaatg aaacacgtaa gtataaaatg acaagtataa gagatgttaa gcctacagat
      301 gtagaagttc tagatgaaca aaaaggtaaa gataaacaat taacattaat tacttgtgat
      361 gattacaatg aaaagacagg cgtttgggaa aaacgtaaaa tctttgtagc tacagaagtc
      421 aaaggctgca tcacgggaga tgcactagtt gccctacccg agggcgagtc ggtacgcatc
      481 gccgacatcg tgccgggtgc gcggcccaac agtgacaacg ccatcgacct gaaagtcctt
      541 gaccggcatg gcaatcccgt gctcgccgac cggctgttcc actccggcga gcatccggtg
      601 tacacggtgc gtacggtcga aggtctgcgt gtgacgggca ccgcgaacca cccgttgttg
      661 tgtttggtcg acgtcgccgg ggtgccgacc ctgctgtgga agctgatcga cgaaatcaag
      721 ccgggcgatt acgcggtgat tcaacgcagc gcattcagcg tcgactgtgc aggttttgcc
      781 cgcgggaaac ccgaatttgc gcccacaacc tacacagtcg gcgtccctgg actggtgcgt
      841 ttcttggaag cacaccaccg agacccggac gcccaagcta tcgccgacga gctgaccgac
      901 gggcggttct actacgcgaa agtcgccagt gtc
//
