LOCUS       sequence-115165-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression vector with Gentamycin selection, based on 
            pBAD18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  TITLE     Juan Tomas plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD18-Gm
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(82..115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
       61 ggcttcccgg ccgacgcgta gataacttcg tatagcatac attatacgaa gttatggatc
      121 cagcttatcg ataccgcggg ccctacgtac gcgtgttaac cggtgagctc ggtacccact
      181 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
      241 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa
      301 attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg
      361 gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacg
//
