LOCUS       sequence-115168-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     246..262
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     247..263
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      291..385
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      488..574
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    625..672
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc aggtgtgggc ggacaataaa
       61 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
      121 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
      181 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
      241 ggggttcgag gtcgacggta tcgataagct agcttaatta gctgagcttg gactcctgtt
      301 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      361 ccgggcgttt tttattggtg agaatccaag ctagactgcg atgagtggca gggcggggcg
      421 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
      481 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      541 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctaggagctt gcggcccgga
      601 cgatgagctc gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag
      661 tataggaact tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaataa
      721 ttctagataa ttcttaggcc acacgttcaa gtgcag
//
