LOCUS       sequence-115169-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             262..498
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     protein_bind    complement(560..607)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
       61 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
      121 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggcagccg ttgtgctggt
      181 gctttctgat agttgttgtg gggtaggcag tcagagctcg atttgcttgt cgccataata
      241 gattcacaag aaggattcga catgggtcaa agtagcgatg aagccaacgc tcccgttgca
      301 gggcagtttg cgcttcccct gagtgccacc tttggcttag gggatcgcgt acgcaagaaa
      361 tctggtgccg cttggcaggg tcaagtcgtc ggttggtatt gcacaaaact cactcctgaa
      421 ggctatgcgg tcgagtccga atcccaccca ggctcagtgc aaatttatcc tgtggctgca
      481 cttgaacgtg tggcctaaga attatctaga attattccat tgagtaagtt tttaagcaca
      541 tcagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
      601 gtacttcaag atccccaatt cgagctcatc
//
