LOCUS       sequence-115172-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..186
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      289..375
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    429..476
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        587..634
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 cgagcgcacc gagggccgcc accacctgtt cctgtagaag ccgaattcca gcacactggc
       61 ggccggtacc aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
      121 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      181 gagaatccaa gctagactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
      241 attggtgccc ttaaacgcct ggggtaatga ctctctagct tgaggcatca aataaaacga
      301 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      361 ctgagtagga caaatccgcc gctaggagct tgcggcccga gctcatgcat gatcgaattg
      421 gggatcttga agtacctatt ccgaagttcc tattctctag aaagtatagg aacttcagag
      481 cgcttttgaa gctgatgtgc ttaaaaactt actcaatgga ataattctag atattcggct
      541 tcgacgcgta actcacgtta agggattttg gtcatgatca gcacgtgttg acaattaatc
      601 atcggcatag tatatcggca tagtataata cgacaaggtg aggaact
//
