LOCUS       sequence-115175-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-gfpmut3
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      23..117
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      220..306
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    360..407
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        518..565
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 taagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
       61 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
      121 agctagactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
      181 cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag
      241 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      301 acaaatccgc cgctaggagc ttgcggcccg agctcatgca tgatcgaatt ggggatcttg
      361 aagtacctat tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga
      421 agctgatgtg cttaaaaact tactcaatgg aataattcta gatattcggc ttcgacgcgt
      481 aactcacgtt aagggatttt ggtcatgatc agcacgtgtt gacaattaat catcggcata
      541 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
      601 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
      661 gctcgggttc t
//
