LOCUS       sequence-115179-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-ecfp
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..27
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(141..159)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     mobile_element  330..495
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gctcagcatg cttaatttct cctctttaat tctagatgtg tgaaattgtt atccgctcac
       61 aattgaatct aagtatcatt gttatccgct cacaagtcaa cactcttttt gataaatttt
      121 ctcgaggtga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      181 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      301 aatggtacct cgcgaaggcc ttgcaggcca accagataag tgaaatctag ttccaaacta
      361 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
      421 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
      481 ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttgggcgct gcattaatga
      541 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      601 actgacccgc tgcgctcggt cgttcggctg cggcgagcgg tatcagagct tatcggccag
      661 cctcgcagag caggattccc gttgagcacc gccaggtgc
//
