LOCUS       sequence-115188-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single vector system expressing NEF allowing myristoylation of 
            recombinant protein produce in Ecoli system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Gluck JM, Hoffmann S, Koenig BW, Willbold D
  TITLE     Single vector system for efficient N-myristoylation of recombinant 
            proteins in E. coli.
  JOURNAL   PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.
  PUBMED    20404920
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-9"; volume: "5(4):e10081. doi"; pages: " 
            10.1371/journal.pone.0010081"
COMMENT     Sequence Label: pETDuet-1∆HisTag_Nef_MCS2
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             631..654
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             655..672
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtggca agtggtcaaa acgtagtatg ggtggatggt ctgctataag ggaaagaatg
       61 agacgagctg agccacgagc tgagccagca gcagatgggg tgggagcagt atctcgagac
      121 ctggaaaaac atggagcaat cacaagtagc aatacagcag ctactaatgc tgattgtgcc
      181 tggctagaag cacaagagga ggaagaggtg ggttttccag tcagacctca ggtaccttta
      241 agaccaatga cttacaaggc agctttagat attagccact ttttaaaaga aaagggggga
      301 ctggaagggc taatttggtc ccaaagaaga caagagatcc ttgatctgtg gatctaccac
      361 acacaaggct acttccctga ttggcagaat tacacaccag ggccagggat cagatatcca
      421 ctgacctttg gatggtgctt caagctagta ccagttgagc cagagaaggt agaagaggcc
      481 aatgaaggag agaacaacag cttgttacac cctatgagcc tacatgggat ggaggacgcg
      541 gagaaagaag tgttagtgtg gaggtttgac agcaaactag catttcatca catggcccga
      601 gagctgcatc cggagtacta caaagactgc ctcgaagttc tgttccaggg gccccatcac
      661 catcatcacc actaa
//
