LOCUS       sequence-115189-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-ecfp
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      27..121
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      224..310
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(372..419)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(460..465,466..482,483..488)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gtaataagct taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga
       61 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
      121 tccaagctag actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
      181 tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc
      241 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      301 taggacaaat ccgccgctag gagcttgcgg cccgagctca tgcatgatcg aattagcttc
      361 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca
      421 agatcccctg attccctttg tcaacagcaa tggatcgaat tgacataagc ctgttcggtt
      481 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      541 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
      601 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      661 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcg
//
