LOCUS       sequence-115195-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             146..157
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    176..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    209..225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     mobile_element  265..430
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            589..697
                     /note="incP origin of transfer"
ORIGIN
        1 tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
       61 actcgtggcc gttcacggtg ccctccatgc gcaccttgaa gcgcatgaac tcggtgatga
      121 cgttctcgga ggaggccatg cttaatttct cctctttaat tctagatgtg tgaaattgtt
      181 atccgctcac aattgaatct aagtatcatt gttatccgct cacaagtcaa cactcttttt
      241 gataaatttt ctcccttgca ggccaaccag ataagtgaaa tctagttcca aactattttg
      301 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
      361 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
      421 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg
      481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      541 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca gagcttatcg gccagcctcg
      601 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
      661 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca
      721 agg
//
