LOCUS       sequence-115197-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KA
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(75..230)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(261..854)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcccct tcctgcagtc agtggtggtg gtggtggtgc
       61 tcgagtgcgg ccgcttgaag ctgccacaag gcaggaacgt tggatggttc ccatcctgcc
      121 aaggaggtgt ggggctgcaa acatttatac gtcttgccgt tatatgtgac caattgtccc
      181 gcagtataag ctgtgttgac ctgccaagcg gatacaccag gatttgtcgt gaggcctgag
      241 ttcagaccgg tgaggccagt agcgtggctg acgaacccgt tcgtgataaa cgcgtggtct
      301 gccgtgtcga cacgaaggct atacaccggc tgcacgccgg cgtcggtgac actggcgact
      361 ttcgcgtagt agaaccgccc gtcggtcagc tcgtcggcga tagcttgggc gtccgggtct
      421 cggtggtgtg cttccaagaa acgcaccagt ccagggacgc cgactgtgta ggttgtgggc
      481 gcaaattcgg gtttcccgcg ggcaaaacct gcacagtcga cgctgaatgc gctgcgttga
      541 atcaccgcgt aatcgcccgg cttgatttcg tcgatcagct tccacagcag ggtcggcacc
      601 ccggcgacgt cgaccaaaca caacaacggg tggttcgcgg tgcccgtcac acgcagacct
      661 tcgaccgtac gcaccgtgta caccggatgc tcgccggagt ggaacagccg gtcggcgagc
      721 acgggattgc catgccggtc aaggactttc aggtcgatgg cgttgtcact gttgggccgc
      781 gcacccggca cgatgtcggc gatgcgtacc gactcgccct cgggtagggc aactagtgca
      841 tctcccgtga tgcagccttt gacttctgta gctacaaaga ttttacgttt ttcccaaacg
      901 cctgtctttt cattgtaatc atcacaagta attaatgtta attgtttatc tttacctttt
      961 tgttcat
//
