LOCUS       sequence-115212-       11533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for generating red fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Rg
FEATURES             Location/Qualifiers
     source          1..11533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(117..911)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(228..247)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     838..857
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     intron          920..1035
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     protein_bind    1874..1998
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2023..2028,2029..2046,2047..2053)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2107..2766
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2173..2192)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3108..3413
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3292..3311
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3454..3578)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3598..4275
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3598..4275
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     3996..4015
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4190..4213
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      4429..4994
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(5025..5042)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5025..5041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5034..5056)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5244..5268
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6568..7197
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7631..8698
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8764..8958
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9194..9216)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9302..9442
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9357..9376
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9457..9474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9628..10216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9708..9727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10303..11097)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    join(11531..11533,1..22)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
       61 gtttttaatg tactgaatta acgccgaata tcgaattcct gcagcccggg ggatcctcag
      121 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
      181 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
      241 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
      301 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
      361 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
      421 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
      481 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
      541 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
      601 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
      661 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
      721 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
      781 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
      841 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
      901 tgttcaatca tggtgatgtc tgctcaagcg gggtagctgt tagtcaagct gcgatgaagt
      961 gggaaagctc gaactgaaag gttcaaagga ataagggatg ggaaggatgg agtatggatg
     1021 tagcaaagta cttacttagg ggaaataaag gttcttggat gggaagatga atatactgaa
     1081 gatgggaaaa gaaagagaaa agaaaagagc agctggtggg gagagcagga aaatatggca
     1141 acaaatgttg gactgacgca acgaccttgt caaccccgcc gacacaccgg gcggacagac
     1201 ggggcaaagc tgcctaccag ggactgaggg acctcagcag gtcgagtgca gagcaccgga
     1261 tgggtcgact gccagcttgt gttcccggtc tgcgccgctg gccagctcct gagcggcctt
     1321 tccggtttca tacaccgggc aaagcaggag aggcacgata tttggacgcc ctacagatgc
     1381 cggatgggcc aattagggag cttacgcgcc gggtactcgc tctacctact tcggagaagg
     1441 tactatctcg tgaatctttt accagatcgg aagcaattgg acttctgtac ctaggttaat
     1501 ggcatgctat ttcgccgacg gctatacacc cctggcttca cattctcctt cgcttactgc
     1561 cggtgattcg atgaagctcc atattctccg atgatgcaat agattcttgg tcaacgaggg
     1621 gcacaccagc ctttccactt cggggcggag gggcggccgg tcccggatta ataatcatcc
     1681 actgcacctc agagccgcca gagctgtctg gccagtggct tattactcag cccttctctc
     1741 tgcgtccgtc cgtctctccg catgccagaa agagtcaccg gtcactgtac agagctctcc
     1801 catatggtcg acctgcaggc ggccgcgaat tcactagtga tttataagct tacgaacgat
     1861 agacatctgg atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     1921 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     1981 acatatccag tcatattggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2041 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     2101 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2161 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2221 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2281 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2341 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2401 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2461 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2521 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2581 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2641 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2701 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2761 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     2821 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2881 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2941 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     3001 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     3061 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     3121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     3181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     3421 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     3481 cagcattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3541 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tagggcccgg gatcctgatg
     3601 gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
     3661 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3721 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     3781 tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3841 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3901 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3961 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4021 ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4081 aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     4141 atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc
     4201 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     4261 tccaccggcg cctgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct
     4321 ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat
     4381 ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc
     4441 tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata cagctcatct
     4501 gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg gcgaagagaa
     4561 gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga tcaacggtcg
     4621 tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat atatttgtct
     4681 ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat gaaaattccg
     4741 tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact ctcaagccta
     4801 caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa gatggtatac
     4861 aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac gccggccgaa
     4921 acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca cttgtttaga
     4981 ggtaatcctt ctttcttcta gacctgcagg catgcaagct tggcactggc cgtcgtttta
     5041 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5101 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5161 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
     5221 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     5281 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     5341 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     5401 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     5461 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     5521 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     5581 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     5641 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     5701 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     5761 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     5821 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     5881 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     5941 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     6001 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     6061 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     6121 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     6181 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     6241 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     6301 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     6361 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     6421 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     6481 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     6541 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     6601 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     6661 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     6721 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     6781 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     6841 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     6901 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     6961 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     7021 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     7081 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     7141 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     7201 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     7261 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     7321 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     7381 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     7441 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     7501 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     7561 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     7621 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     7681 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     7741 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     7801 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     7861 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     7921 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     7981 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     8041 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     8101 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     8161 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     8221 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     8281 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     8341 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     8401 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     8461 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     8521 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     8581 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     8641 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     8701 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     8761 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     8821 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     8881 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     8941 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     9001 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     9061 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     9121 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     9181 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9241 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9301 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     9361 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9421 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10261 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
    10321 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    10381 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    10441 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    10501 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    10561 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    10621 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    10681 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    10741 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    10801 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    10861 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    10921 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    10981 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    11041 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    11101 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    11161 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    11221 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    11281 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    11341 atcatccgtg tttcaaaccc ggnnncagct tagttgccgt tcttcnnncg aatagcatcg
    11401 gtaacatnnn gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11461 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11521 ggctggctgg tgg
//
