LOCUS       sequence-115214-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      91..185
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      288..374
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(500..547)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(588..593,594..610,611..616)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gagcgcaccg agggccgcca ccacctgttc ctgtagaagc cgaattccag cacactggcg
       61 gccggtacca agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct
      121 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
      181 agaatccaag ctagactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
      241 ttggtgccct taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa
      301 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      361 tgagtaggac aaatccgccg ctaggagctt gcggcccgcc ttcgcgaggt accgggccca
      421 agcttctcga ggaattcctg cagcccgggg gatccactag tgagctcatg catgatcgaa
      481 ttagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
      541 gaacttcaag atcccctgat tccctttgtc aacagcaatg gatcgaattg acataagcct
      601 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
      661 tgaccgaacg cagcggtggt aacggcgcag tggcgg
//
