LOCUS       sequence-115215-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18PV-mini-Tn7T
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     294..310
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     295..311
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      339..433
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      536..622
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcttcgctat tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt
       61 gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga
      121 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
      181 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
      241 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt
      301 cgacggtatc gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt
      361 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      421 tattggtgag aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa
      481 ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat
      541 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      601 cgctctcctg agtaggacaa atccgccgct aggagcttgc ggcccggacg atatcatgca
      661 tgagctcact agtggatccc ccgggctgca ggaattcctc gagaagcttg ggcccggtac
      721 ctcgcgaagg ccttgcaggc caaccagata agtgaaatct ag
//
