LOCUS       sequence-115216-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18PV-mini-Tn7T
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  163..328
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(448..534)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(637..731)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(759..775)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgcgttatca gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg ggtcagtgag
       61 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      121 ttaatgcagc gcccaaacat aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag
      181 ttgggaactg ggaggggtgg aaatggagtt tttaaggatt atttagggaa gagtgacaaa
      241 atagatggga actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag
      301 tttggaacta gatttcactt atctggttgg cctgcaaggc cttcgcgagg taccgggccc
      361 aagcttctcg aggaattcct gcagcccggg ggatccacta gtgagctcat gcatgatatc
      421 gtccgggccg caagctccta gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
      481 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
      541 caagctagag agtcattacc ccaggcgttt aagggcacca ataactgcct taaaaaaatt
      601 acgccccgcc ctgccactca tcgcagtcta gcttggattc tcaccaataa aaaacgcccg
      661 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
      721 caacaggagt ccaagctcag ctaattaagc tagcttatcg ataccgtcga cctcg
//
