LOCUS       sequence-115217-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     oriT            174..282
                     /note="incP origin of transfer"
     mobile_element  441..606
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tgttctttcc tgcgttatca ggggattcct taaggtatac tttccgctgc ataaccctgc
       61 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
      121 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
      181 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
      241 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgataagctc tgataccgct
      301 cgccgcagcc gaacgaccga gcgcagcggg tcagtgagcg aggaagcgga agagcgccca
      361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcgc ccaaacataa
      421 caggaagaaa aatgccccgc tgtgggcgga caaaatagtt gggaactggg aggggtggaa
      481 atggagtttt taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc
      541 gtcgtaagct aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat
      601 ctggttggcc tgcaaggcct tcgcgaggta ccgggcccaa gcttctcgag gaattcctgc
      661 agcccggggg atccactagt gagctcatgc atgatcgagc tcgaattagc ttcaaaagcg
      721 ctctgaagtt cctatacttt ctagagaata
//
