LOCUS       sequence-115218-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single vector system expressing hNMT1 (81-496 amino acids) and NEF 
            allowing myristoylation of recombinant protein produce in Ecoli 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Gluck JM, Hoffmann S, Koenig BW, Willbold D
  TITLE     Single vector system for efficient N-myristoylation of recombinant 
            proteins in E. coli.
  JOURNAL   PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.
  PUBMED    20404920
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-9"; volume: "5(4):e10081. doi"; pages: " 
            10.1371/journal.pone.0010081"
COMMENT     Sequence Label: pETDuet-1∆HisTag_hNMT_Nef
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acaattcccc atcttagtat attagttaag tataagaagg agatatacat atgggtggca
       61 agtggtcaaa acgtagtatg ggtggatggt ctgctataag ggaaagaatg agacgagctg
      121 agccacgagc tgagccagca gcagatgggg tgggagcagt atctcgagac ctggaaaaac
      181 atggagcaat cacaagtagc aatacagcag ctactaatgc tgattgtgcc tggctagaag
      241 cacaagagga ggaagaggtg ggttttccag tcagacctca ggtaccttta agaccaatga
      301 cttacaaggc agctttagat attagccact ttttaaaaga aaagggggga ctggaagggc
      361 taatttggtc ccaaagaaga caagagatcc ttgatctgtg gatctaccac acacaaggct
      421 acttccctga ttggcagaat tacacaccag ggccagggat cagatatcca ctgacctttg
      481 gatggtgctt caagctagta ccagttgagc cagagaaggt agaagaggcc aatgaaggag
      541 agaacaacag cttgttacac cctatgagcc tacatgggat ggaggacgcg gagaaagaag
      601 tgttagtgtg gaggtttgac agcaaactag catttcatca catggcccga gagctgcatc
      661 cggagtacta caaagactgc ctcgaagttc tgttc
//
