LOCUS       sequence-115220-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA-KQ
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(60..215)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(246..839)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 gcagccggat ccccttcctg cagtcagtgg tggtggtggt ggtgctcgag tgcggccgct
       61 tgaagctgcc acaaggcagg aacgttggat ggttcccatc ctgccaagga ggtgtggggc
      121 tgcaaacatt tatacgtctt gccgttatat gtgaccaatt gtcccgcagt ataagctgtg
      181 ttgacctgcc aagcggatac accaggattt gtcgtgaggc ctgagttcag accggtgagg
      241 ccagtagcgt ggctgacgaa cccgttcgtg ataaacgcgt ggtctgccgt gtcgacacga
      301 aggctataca ccggctgcac gccggcgtcg gtgacactgg cgactttcgc gtagtagaac
      361 cgcccgtcgg tcagctcgtc ggcgatagct tgggcgtccg ggtctcggtg gtgtgcttcc
      421 aagaaacgca ccagtccagg gacgccgact gtgtaggttg tgggcgcaaa ttcgggtttc
      481 ccgcgggcaa aacctgcaca gtcgacgctg aatgcgctgc gttgaatcac cgcgtaatcg
      541 cccggcttga tttcgtcgat cagcttccac agcagggtcg gcaccccggc gacgtcgacc
      601 aaacacaaca acgggtggtt cgcggtgccc gtcacacgca gaccttcgac cgtacgcacc
      661 gtgtacaccg gatgctcgcc ggagtggaac agccggtcgg cgagcacggg attgccatgc
      721 cggtcaagga ctttcaggtc gatggcgttg tcactgttgg gccgcgcacc cggcacgatg
      781 tcggcgatgc gtaccgactc gccctcgggt agggcaacta gtgcatctcc cgtgatgcag
      841 cctttgactt ctgtagctac aaagatttta cgtttttccc aaacgcctgt cttttc
//
