LOCUS       sequence-115222-       11720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for generating green fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Gh
FEATURES             Location/Qualifiers
     source          1..11720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    100..274
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(305..1330)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    1811..1832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1847..1852,1853..1870,1871..1877)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1885..1901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1890..1912
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1909..1925
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1909..1925
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2022..2146
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2171..2176,2177..2194,2195..2201)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2255..2914
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2321..2340)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3256..3561
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3440..3459
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3602..3726)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3746..3748,3749..3751,3752..4465)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3746..3748,3749..3751,3752..4462)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3791..3812)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4052..4071)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4399..4420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4619..5184
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(5212..5229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5212..5228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5221..5243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5431..5455
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6755..7384
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7818..8885
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8951..9145
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9381..9403)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9489..9629
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9544..9563
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9644..9661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9815..10403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9895..9914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10490..11284)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    join(11718..11720,1..22)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
       61 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
      121 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
      181 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
      241 atctgggaac tactcacaca ttattatgga gaaactcgaa actggttccc ggtcggcatc
      301 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
      361 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
      421 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
      481 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
      541 cccggaggcg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
      601 gctccataca agccaaccac ggcctccaga agaggatgtt ggcgacctcg tattgggaat
      661 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
      721 cattgttgga gccgaaatcc gcatgcacga ggtgccggac ttcggggcag tcctcggccc
      781 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
      841 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
      901 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
      961 cagcgatcgc atccatggcc tccgcgaccg gctggagaac agcgggcagt tcggtttcag
     1021 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     1081 tgaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     1141 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     1201 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     1261 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     1321 gctttttcat ttggatgctt gggtagaata ggtaagtcag attgaatctt gaaataaagg
     1381 gaggaagggc gaacttaaga aggtatgacc gggtcgtcca cttaccttgc ttgacaaacg
     1441 caccaagtta tcgtgcacca agcagcagat gataataatg tcctcgttcc tgtctgctaa
     1501 taagagtcac acttcgagcg ccgccgctac tgctacaagt ggggctgatc tgaccagttg
     1561 cctaaatgaa ccatcttgtc aaacgacaca aattttgtgc tcaccgcctg gacgactaaa
     1621 ccaaaatagg cattcattgt tgacctccac tagctccagc caagcccaaa aaatgctcct
     1681 tcaatatcag ttttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
     1741 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1801 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     1861 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     1921 atgacatgat tacgaattcg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc
     1981 actagtgatt tataagctta cgaacgatag acatctggat cacaagtttg tacaaaaaag
     2041 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     2101 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     2161 caccccaggc tttacacttt atgcttccgg ctcgtataac gtgtggattt tgagttagga
     2221 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     2281 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2341 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2401 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2461 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2521 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2581 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2641 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2701 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2761 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2821 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2881 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     2941 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     3001 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     3061 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     3121 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     3181 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     3241 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     3301 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3361 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3421 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3481 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3541 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3601 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3661 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3721 agtggtgata gggcccggga tcctgatggt gagcaagggc gaggagctgt tcaccggggt
     3781 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3841 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3901 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3961 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4021 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4081 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4141 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4201 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4261 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4321 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4381 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4441 cggcatggac gagctgtaca agtgagatcc acttaacgtt actgaaatca tcaaacagct
     4501 tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg
     4561 tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg
     4621 atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata
     4681 cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg
     4741 gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga
     4801 tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat
     4861 atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat
     4921 gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact
     4981 ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa
     5041 gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac
     5101 gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca
     5161 cttgtttaga ggtaatcctt ctttctagac ctgcaggcat gcaagcttgg cactggccgt
     5221 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5281 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5341 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     5401 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     5461 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     5521 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     5581 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     5641 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     5701 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     5761 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     5821 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     5881 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     5941 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     6001 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     6061 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     6121 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     6181 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     6241 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     6301 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     6361 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     6421 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     6481 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     6541 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     6601 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     6661 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     6721 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     6781 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     6841 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     6901 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     6961 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     7021 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     7081 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     7141 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     7201 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     7261 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     7321 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     7381 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     7441 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     7501 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     7561 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     7621 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     7681 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     7741 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     7801 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     7861 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     7921 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     7981 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     8041 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     8101 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     8161 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     8221 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     8281 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     8341 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     8401 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     8461 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     8521 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     8581 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     8641 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     8701 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     8761 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     8821 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     8881 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     8941 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     9001 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     9061 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     9121 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     9181 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     9241 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     9301 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     9361 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9421 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9481 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     9541 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9601 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     9661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    10261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10441 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    10501 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    10561 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    10621 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    10681 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    10741 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    10801 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    10861 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    10921 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    10981 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    11041 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    11101 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    11161 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    11221 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    11281 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    11341 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    11401 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    11461 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    11521 ccgcgagatc atccgtgttt caaacccggn nncagcttag ttgccgttct tcnnncgaat
    11581 agcatcggta acatnnngag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    11641 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    11701 agctgttggc tggctggtgg
//
