LOCUS       sequence-115231-        1177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""flipped and extended"" sgRNA backbone (Chen et al., 2013) 
            following a C. elegans U6 promoter (Friedland et al., 2013). For 
            Gibson cloning locus-specific sgRNA sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Katic I, Xu L, Ciosk R
  TITLE     CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of 
            Templates for Homology-Mediated Repair and Knock-Ins by 
            Homology-Independent DNA Repair.
  JOURNAL   G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273.
  PUBMED    26044730
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273."
COMMENT     Sequence Label: pIK198
FEATURES             Location/Qualifiers
     source          1..1177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(433..455)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     555..574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     768..790
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     782..799
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     783..799
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
      421 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      481 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      541 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      601 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
      661 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      721 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      781 ttgtaaaacg acggccagtg ctccaagaac tcgtacaaaa atgctctgaa gtaggtctcg
      841 agatcaatac tgggaagacg aaagtcttgc gaaaccgatt cgctgacccc agtaaagtct
      901 acttcggtag cccttccccc accacccagc tcgacgacgt cgacgagtac atctacctcg
      961 gtcgtcaaat caacgcccaa aacaacttga tgccggaaat ccaccgaaga cgtcgagcag
     1021 cctgggctgc attcaatgga atcaagaatg ccaccgactc catcaccgac aagaagattc
     1081 gtgcgaatct gttcgactca attgtccttc cagcgctcac ctacgtttca gaagcctgga
     1141 cattcaccaa agctctatcc gaacgagtac gaatcac
//
