LOCUS       sequence-115235-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 
            (eft-3) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Katic I, Xu L, Ciosk R
  TITLE     CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of 
            Templates for Homology-Mediated Repair and Knock-Ins by 
            Homology-Independent DNA Repair.
  JOURNAL   G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273.
  PUBMED    26044730
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273."
COMMENT     Sequence Label: pIK127
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..650
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    659..683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(716..744)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atctttgtat agaaaagttg gcacctttgg
       61 tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa tgaatttttc
      121 ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata aactgccaga
      181 gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg tacattttcg
      241 tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt gggccttgtg
      301 aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt tggtttattt
      361 tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat ttataatgag
      421 gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat ttttatttta
      481 ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata tttttttaat
      541 ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc tctaccgtcc
      601 gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca ctttgctcac
      661 aagtttgtac aaaaaagcag gctccatgag taaaggagaa gaacttttca ctggagttgt
      721 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      781 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      841 actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg attatttaaa
      901 ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct cgagataccc
      961 agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1021 aagaactata tttttcaaag atgacgggaa ctaca
//
