LOCUS       sequence-115236-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA2woH
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcgcacaat gtgcgccatt tttcacttca caggtcgttt aaacttattt gacttctgta
       61 gctacaaaga ttttacgttt ttcccaaacg cctgtctttt cattgtaatc atcacaagta
      121 attaatgtta attgtttatc tttacctttt tgttcatcta gaacttctac atctgtaggc
      181 ttaacatctc ttatacttgt cattttatac ttacgtgttt cattaccaac tttaaagtac
      241 accatactac cttttttggc tgctttaaga tttgtaaatt gatagttcgg acggtcaatg
      301 aaagtgtgtc ctgcaattga aatattttga tcatctagtg aggcattttc ctttgcaaag
      361 cttacacctc tatttaattg ttcaggtgtt gctggtcctg gatatactgg ttctttaata
      421 tcagcatctg gaatttcaat atagcctgcc actttcgatt tatctttcgg aatttgaggt
      481 ttagcttggg tacccagatc tgggctgtcc atgtgctggc gttc
//
