LOCUS       sequence-115237-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 
            (eft-3) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Katic I, Xu L, Ciosk R
  TITLE     CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of 
            Templates for Homology-Mediated Repair and Knock-Ins by 
            Homology-Independent DNA Repair.
  JOURNAL   G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273.
  PUBMED    26044730
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 
            10.1534/g3.115.019273."
COMMENT     Sequence Label: pIK127
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(70..393)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    398..422
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(883..903)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcag gcctctgcag tcgacgggcc cgggatccga
       61 tagttgtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
      121 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
      181 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
      241 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
      301 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
      361 gagaagaagg gaatgcttga aaggattttg catttatccc actttgtaca agaaagctgg
      421 gtttacttgc tggaagtgta cttggtgacg gccttggttc cctcagacac ggcgtgcttg
      481 gcaagttctc ctgggagaat caaacggaca gcggtttgaa tttcgcggga tgagatcgtt
      541 gagcgtttgt tgtaatgagc aagacgggaa gcttccgaag cgatgcgttc gaatacatcg
      601 ttgacgaagg agttcatgat agacatggcc ttggaggaga ctccggtgtc tgggtgaact
      661 tgcttgagaa cacggtagat gtagacggag tacgattcct tgcgggcatg acgtctcttc
      721 tttccgtcct ttggcttggc aacgacggtc ttggcggcct tcttggctcc cttggcagat
      781 ggctttggtg gactagttct agagcggccg ccaccggtgg agctccaccg atcccccggg
      841 catttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg
      901 accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa
      961 tggttgtctg gtaaaaggac agggccatcg ccaattggag
//
