LOCUS       sequence-115238-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Staphylococcus aureus sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtAwoH
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..74
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(709..728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatga aagaaaccgc tgctgctaaa ttcgaacgcc
       61 agcacatgga cagcccagat ctgggtaccc aagctaaacc tcaaattccg aaagataaat
      121 cgaaagtggc aggctatatt gaaattccag atgctgatat taaagaacca gtatatccag
      181 gaccagcaac acctgaacaa ttaaatagag gtgtaagctt tgcagaagaa aacgaatcac
      241 tagatgatca aaatatttca attgcaggac acactttcat tgaccgtccg aactatcaat
      301 ttacaaatct taaagcagcc aaaaaaggta gtatggtgta ctttaaagtt ggtaatgaaa
      361 cacgtaagta taaaatgaca agtataagag atgttaagcc tacagatgta gaagttctag
      421 atgaacaaaa aggtaaagat aaacaattaa cattaattac ttgtgatgat tacaatgaaa
      481 agacaggcgt ttgggaaaaa cgtaaaatct ttgtagctac agaagtcaaa taagtttaaa
      541 cgacctgtga agtgaaaaat ggcgcacatt gtgcgacatt ttttttgtct gccgtttacc
      601 gctactgcgt cacggatctc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      721 tcttcccttc cg
//
